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Asian-Australas J Anim Sci > Volume 21(8); 2008 > Article
Animal Products
Asian-Australasian Journal of Animal Sciences 2008;21(8): 1220-1225.
DOI: https://doi.org/10.5713/ajas.2008.70698    Published online July 1, 2008.
Effect of Genistein on Antioxidative Defence System and Membrane Fluidity in Chick Skeletal Muscle Cells
Z. Y. Jiang*, S. Q. Jiang, Y. C. Lin, X. Y. Ma, P. B. Xi, T. Cao, X. Q. Wang
Correspondence:  Z. Y. Jiang,
Abstract
This study was conducted to investigate the protective effect of genistein on the antioxidative defence system and membrane fluidity in chick skeletal muscle cells after supplementation with 0, 20, 40, and 80 mol/L genistein in 50 mol/L FeSO4/ H2O2 treated cells for 24 h. Genistein supplementation recovered the decreased activity of total superoxide dismutase induced by FeSO4/ H2O2, significantly increased glutathione peroxidase activity (p<0.05) and decreased malondialdehyde production (p<0.05). The treatment of 80 mol/L genistein in FeSO4/H2O2 treated cells decreased the secretion of creatine kinase (p<0.05). Fluorescence polarization values and microviscosities observed with FeSO4/H2O2 treated cells were significantly higher than those observed with no FeSO4/H2O2 treated cells. The addition of 80 mol/L genistein improved the increased fluorescence polarization value (p<0.05) caused by FeSO4/H2O2 treatment. The microviscosity value was significantly decreased by adding genistein (p<0.05). In conclusion, genistein protected skeletal muscle cells from oxidative damage by improving antioxidative status and membrane fluidity.
Keywords: Genistein; Lipid Peroxidation; Membrane Fluidity; Antioxidation; Chick Skeletal Muscle Cells


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