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Asian-Australas J Anim Sci > Volume 20(10); 2007 > Article
Animal Products
Asian-Australasian Journal of Animal Sciences 2007;20(10): 1612-1617.
DOI: https://doi.org/10.5713/ajas.2007.1612    Published online August 30, 2007.
Developmental Proteomic Profiling of Porcine Skeletal Muscle during Postnatal Development
Nam-Kuk Kim, Jong-Hyun Lim, Min-Jin Song, Oun-Hyun Kim, Beom-Young Park, Myung-Jick Kim, In-Ho Hwang, Chang-Soo Lee
Abstract
In this study, we have compared the skeletal muscle proteome at various stages of porcine postnatal development. Korean native pigs were divided into five postnatal stages of 30, 70, 130, 170 and 300 d and their loin muscles were analyzed for muscle proteome by using two-dimensional electrophoresis and mass spectrometry. We found 5 proteins showing a consistent pattern during skeletal muscle growth. Four proteins were identified as myosin light chain 1 slow-twitch (MLC1sa) isoform, troponin T, triosephosphate isomerase (TIP) and DJ-1 protein. The remaining protein was not identified. Two muscle fiber proteins of MLC1sa isoform and troponin T showed a high expression level at an early postnatal stage and then their levels were decreased markedly during growth stages. On the other hand, the expression of TIP and DJ-1 protein, which are well known as catalysis enzyme and antioxidant-related protein, respectively, were linearly increased during growth stages. Thus, the stage-related muscle proteins may be useful as parameters for understanding the developmental characteristics of biochemical and physiological properties in Korean native pig skeletal muscle.
Keywords: Muscle Proteome; Growth; Two-dimensional Electrophoresis


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