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Asian-Australas J Anim Sci > Volume 20(10); 2007 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2007;20(10): 1473-1477.
DOI: https://doi.org/10.5713/ajas.2007.1473    Published online August 30, 2007.
Full-length cDNA, Expression Pattern and Association Analysis of the Porcine FHL3 Gene
Bo Zuo, YuanZhu Xiong, Hua Yang, Jun Wang
Abstract
Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.
Keywords: Pig; FHL3; Meat Quality; In silico Mapping


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