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Asian-Australas J Anim Sci > Volume 20(9); 2007 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2007;20(9): 1349-1353.
DOI: https://doi.org/10.5713/ajas.2007.1349    Published online August 30, 2007.
Expression Characterization, Polymorphism and Chromosomal Location of the Porcine Calsarcin-3 Gene
Heng Wang, Shulin Yang, Zhonglin Tang, Yulian Mu, Wentao Cui, Kui Li
Abstract
Calcineurin is a calmodulin dependent protein that functions as a regulator of muscle cell growth and function. Agents capable of interacting with calcineurin could have important applications in muscle disease treatment as well as in the improvement of livestock production. Calsarcins comprise a family of muscle-specific calcineurin binding proteins which play an important role in modulating the function of calcineurin in muscle cells. Recently, we described the first two members of the calsarcin family (calsarcin-1 and calsarcin-2) in the pig. Here, we characterized the third member of the calsarcin family, calsarcin-3, which is also expressed specifically in skeletal muscle. However, unlike calsarcin-1 and calsarcin-2, the calsarcin-3 mRNA expression in skeletal muscle kept rising throughout the prenatal and postnatal development periods. In addition, radiation hybrid mapping indicated that porcine calsarcin-3 mapped to the distal end of the q arm of pig chromosome 2 (SSC2). A C/T single nucleotide polymorphism site in exon 5 was genotyped using the denaturing high performance liquid chromatography (DHPLC) method and the allele frequencies at this locus were significantly different among breeds.
Keywords: Expression; Localization; Polymorphism; Calsarcin-3; Porcine


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