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Asian-Australasian Journal of Animal Sciences 2007;20(7): 1115-1119.
DOI: https://doi.org/10.5713/ajas.2007.1115    Published online June 27, 2007.
2, 4-Thiazolidindion Induced Plasticity of Myoblast (C2C12) and Satellite Cells (Porcine) - A Comparative Study
N. K. Singh, H. S. Chae*, I. H. Hwang, Y. M. Yoo, C. N. Ahn, H. J. Lee, H. J. Park, H. Y. Chung
Correspondence:  H. S. Chae,
Abstract
This study was conducted to determine the difference between satellite cells (porcine) and myoblasts (C2C12) in their differentiation under the influence of 2, 4-thiazolidindion. C2C12 myoblast cells and porcine satellite cells (isolated from 10 d old LandraceDuroc piglets) were grown to absolute confluency. Post confluent cells (day 0) were further exposed to adipogenic induction medium along with 2, 4-thiazolidindion (8 M) for 2 d. Thereafter, cells were exposed to 2, 4-thiazolidindion alone every 2 d till day 10 and analysed. The control was cultured in differentiation medium without any treatment. Increased (p<0.05) expression of transcriptional factors i.e. C/EBP- and PPAR- and transition of cells to adipocyte morphology was noticed from 2 d and 4 d onwards in satellite cells (Porcine) and myoblasts (C2C12) respectively. Myogenesis was observed to be suppressed completely in case of satellite cells compared to myoblasts in response to 2, 4-thiazolidindion. Pax-7 (transcriptional factor) appeared as a sole entity to satellite cells only, as it was not identified in case of myoblasts. Although both the cells were converting to adipoblasts, the degree of their conversion was different in response to 2, 4-thiazolidindion. Therefore, the hypothesis that satellite cells contribute various domains to the growing myoblasts appeared obscured and found to be dependent on the proliferative energy/or degree of fusion. However, it revealed satellite cells as currency to myoblasts/muscle.
Keywords: Adipogenesiss; Adipogenesis; Myoblast (C2C12); Satellite Cells (Porcine); Pax-7; 2, 4-Thiazolidindion
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