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Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2007;20(7): 1015-1022.
DOI: https://doi.org/10.5713/ajas.2007.1015    Published online June 27, 2007.
Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
D. K. Jeong*, Michael D. Griswold
Correspondence:  D. K. Jeong,
Abstract
The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be realized if stem cells could be cultured, transfected with unique genes, and then transplanted into recipient testes. We developed a culture system that supported long-term viability of SSCs. We used a retrovirus vector (pMSCV including -galactosidase) to stably transfect spermatogonia following long-term culture using the system developed. Expression of the reporter gene -galactosidase controlled by the retroviral vector was stable in long-term cultured SSCs. We confirmed the retroviral-mediated -galactsidase gene could be expressed in germ cells in recipient mice following SSCs transplantation.
Keywords: Retrovirus; Gene Transfer; Electroporation; Stem Cell Medium
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