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Asian-Australas J Anim Sci > Volume 20(6); 2007 > Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2007;20(6): 856-865.
DOI: https://doi.org/10.5713/ajas.2007.856    Published online May 2, 2007.
Development and Characterization of a Specific Anti-Caveolin-1 Antibody for Caveolin-1 Functional Study in Human, Goat and Mouse
Meng-Wei Ke, Yan-Nian Jiang, Yi-Hung Li, Ting-Yu Tseng, Ming-Shung Kung, Chiun-Sheng Huang, Winston Teng-Kuei Cheng, Jih-Tay Hsu, Yu-Ten Ju*
Correspondence:  Yu-Ten Ju,
Abstract
Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-1 protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-1 and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells.
Keywords: Caveolin-1; Peptide Antibody; Breast Cancer; Goat; Cancer Marker


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