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Asian-Australas J Anim Sci > Volume 20(6); 2007 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2007;20(6): 817-820.
DOI: https://doi.org/10.5713/ajas.2007.817    Published online May 2, 2007.
Association of Sequence Variations in DGAT 1 Gene with Economic Traits in Hanwoo (Korea Cattle)
H. S. Kong, J. D. Oh, J. H. Lee, D. H. Yoon, Y. H. Choi, B. W. Cho, H. K. Lee, G. J. Jeon*
Correspondence:  G. J. Jeon,
Abstract
The effects of diacylglycerol O-acyltransferase (DGAT1) candidate gene polymorphism on the economic traits of Hanwoo were studied. Through sequencing analysis, two polymorphism sites at K232A and T11993C were established and were analyzed by PCR-RFLP. The PCR-RFLP analysis for K232A showed that the frequencies of alleles K and A were 0.75 and 0.25, respectively, and the frequencies of genotypes for K/K, K/A and A/A were estimated as 0.509, 0.491 and 0, respectively. In the PCR-RFLP analysis for T11993C, we found allele frequencies of 0.773 and 0.227 for T and A, respectively, and 0.546, 0.454 and 0 for the T/T, T/C and C/C genotype frequencies, respectively. No significant effects on economic traits in Hanwoo were found in the separate analysis of K232A and T11993C polymorphisms, but the interaction between K232A and T11993C showed a significant effect (p<0.005) on marbling score. The DGAT1 candidate gene was found to have a significant effect not only on milk yield and component traits but also on the metabolism of intramuscular fat.
Keywords: DGAT1 Gene; Single Nucleotide Polymorphism (SNP); Hanwoo


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