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Asian-Australas J Anim Sci > Volume 20(5); 2007 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2007;20(5): 633-637.
DOI: https://doi.org/10.5713/ajas.2007.633    Published online March 5, 2007.
Molecular Characterisation of Nilagiri Sheep (Ovis aries) of South India Based on Microsatellites
Haris Girish, S. N. Sivaselvam*, S. M. K. Karthickeyan, R. Saravanan
Correspondence:  S. N. Sivaselvam,
Abstract
Genetic variation in Nilagiri sheep, the only apparel wool breed in South India was studied using 25 FAO recommended ovine-specific microsatellite markers. The number of observed alleles ranged from 3 to 8 with a mean of 5 across all loci. The size of alleles ranged from 72 to 228 bp. The frequency of alleles ranged from 0.0104 to 0.5781. In total, 125 alleles were observed at the 25 loci studied. The effective number of alleles ranged from 2.18 to 6.49. The mean number of effective alleles was 3.84 across all loci. All the 25 loci were found to be highly polymorphic. The PIC values ranged from 0.4587 to 0.8277 with a mean of 0.6485. Of 25 microsatellites studied, 17 were in Hardy-Weinberg Equilibrium proportions. The observed heterozygosity ranged from 0.4222 to 1.000 with a mean value of 0.7610 whereas the expected heterozygosity ranged from 0.5415 to 0.8459 with a mean value of 0.7213. Except six loci, the other loci revealed negative within-population inbreeding estimates (FIS) indicating excess of heterozygotes in the population of Nilagiri sheep.
Keywords: Genetic Characterisation; Nilagiri Sheep; Microsatellites; PIC; Inbreeding Estimates


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