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Asian-Australas J Anim Sci > Volume 19(9); 2006 > Article
Animal Products
Asian-Australasian Journal of Animal Sciences 2006;19(9): 1335-1341.
DOI: https://doi.org/10.5713/ajas.2006.1335    Published online August 2, 2006.
Antioxidative and Probiotic Properties of Lactobacillus gasseri NLRI-312 Isolated from Korean Infant Feces
H. S. Kim*, S. G. Jeong, J. S. Ham, H. S. Chae, J. M. Lee, C. N. Ahn
Correspondence:  H. S. Kim,
Abstract
We selected a Lactobacillus spp. from Korean healthy infant feces based upon their antioxidant activity. This strain was identified as Lactobacillus gasseri by 16S rDNA sequencing, and named Lactobacillus gasseri NLRI-312. In the present study, we investigate the protective effect of this strain on the H2O2 induced damage to cellular membrane lipid and DNA in Jurkat cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA (malondialdehyde) was measured, and DNA damage was tested by the comet assay. We also examined probiotic properties including tolerance to acid and bile, antibiotic resistance. From the results obtained, the supplementation of Jurkat cells with NLRI-312 decreased in DNA damage, while no effect was shown on MDA decrease. In probiotic properties, this strain was resistance to both acid and bile, showed considerably higher survival when incubated in pH 2 or 1% bile salts (w/v). We concluded that the NLRI-312 could be used as potential probiotic bacteria, with the effect of reducing DNA damage induced by H2O2.
Keywords: Lactobacillus gasseri; Antioxidant; Probiotic; DNA Damage; Jurkat Cell


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