Go to Top Go to Bottom
Asian-Australas J Anim Sci > Volume 19(9); 2006 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2006;19(9): 1240-1244.
DOI: https://doi.org/10.5713/ajas.2006.1240    Published online August 2, 2006.
A Y-linked SNP in SRY Gene Differentiates Chinese Indigenous Swamp Buffalo and Introduced River Buffalo
Yi "Zhang,", Dongxiao Sun, Ying Yu, Yuan Zhang*
Correspondence:  Yuan Zhang,
Abstract
The complete coding region sequence of the SRY gene in Chinese swamp buffalo was determined by PCR product sequencing. Comparison of swamp and river buffalo SRY gene sequences revealed a single nucleotide polymorphism (SNP, C/G) at the 202 bp site of the coding region. Further, a total of 124 male domestic buffaloes were genotyped at this SNP site using the PCR-SSCP method, and it was found that all Chinese indigenous swamp buffaloes had a guanine (G) at this site, while introduced river buffaloes and crossbred buffaloes showed a cytosine (C). Our findings suggested that this Y-linked SNP displayed type-specific alleles differentiating swamp and river buffaloes, and could be used as an effective marker to detect crossbreeding of swamp buffaloes with introduced river buffaloes in native buffalo populations, and thereby assess genetic diversity status and make proper conservation decisions for indigenous swamp buffaloes. In addition, this SNP can be potentially applied in the study of Asian water buffalo phylogeny from a male perspective.
Keywords: Swamp buffalo; River buffalo; SRY; SNP; Genetic diversity; Conservation
TOOLS
METRICS Graph View
  • 2 Crossref
  • 4 Scopus
  • 1,941 View
  • 20 Download
Related articles


Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2019 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Close layer
prev next