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Asian-Australas J Anim Sci > Volume 19(7); 2006 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2006;19(7): 953-957.
DOI: https://doi.org/10.5713/ajas.2006.953    Published online May 25, 2006.
Molecular Characterization and Expression Patterns of Porcine Eukaryotic Elongation Factor 1 A
H. L. Wang, H. Wang, Z. M. Zhu, S. L. Yang, S. T Feng, K. Li*
Correspondence:  K. Li,
Abstract
The eukaryotic elongation factor 1 A (EEF1A) participates in protein synthesis by forming the eEF1A쨌GTP쨌tRNA complex to deliver aminoacyl-tRNA to the A site of ribosomes. This study described cDNA sequences and partial genomic structure of porcine EEF1A1. The porcine EEF1A1 gene encoded a protein with 462 amino acids, which shared complete homology with human, chimpanzee and dog. The temporal expression pattern showed the diversity of EEF1A1 level in mRNA was relatively minor in prenatal embryo skeletal muscle, however, the expression decreased during aging after birth in skeletal muscle of the Chinese Tongcheng pig. The spatial expression patterns indicated that the gene expressed in skeletal muscle, heart, lung, liver, kidney, fat and spleen. In addition, we assigned the gene to porcine chromosome 1 using a radiation hybrid panel.
Keywords: EEF1A1; Expression; Chromosome; Genome; Porcine


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