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Asian-Australas J Anim Sci > Volume 18(11); 2005 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2005;18(11): 1519-1523.
DOI: https://doi.org/10.5713/ajas.2005.1519    Published online December 2, 2005.
Association Analyses with Carcass Traits in the Porcine KIAA1717 and HUMMLC2B Genes
D. Q. Xu, Y. Z. Xiong, M. Liu, J. Lan, X. F. Ling, C. Y. Deng, S. W. Jiang
Abstract
By screening a subtracted cDNA library constructed with mRNA obtained from the longissimus dorsi muscles of F1 hybrids Landrace횞Yorkshire and their Yorkshire female parents, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. In the present work we investigated two SNPs, one (C1354T) at the 3` untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, in a resource population derived from crossing Chinese Meishan and Large White pig. The selected pigs were genotyped by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with thorax-waist backfat thickness (p<0.05), buttock backfat thickness (p<0.05), average backfat thickness (p<0.05), loin eye height (p<0.05), loin eye area (p<0.05), carcass length to 1st spondyle (p<0.01) and carcass length to 1st rib (p<0.01). HUMMLC2B A345G were significantly associated with loin eye width (p<0.05), loin eye area (p<0.05). Further studies are needed to confirm these preliminary results.
Keywords: H3-K4-Specific Methyltransferase; Myosin Regulatory Light Chain; Pigs; Polymorphism; Carcass Traits


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