• Home
  • E-Submission
  • Sitemap
  • Contact Us
Asian-Australas J Anim Sci. Search

CLOSE


Go to Top Go to Bottom
Asian-Australas J Anim Sci > Volume 18(8); 2005 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2005;18(8): 1080-1087.
DOI: https://doi.org/10.5713/ajas.2005.1080    Published online November 29, 2005.
cDNA Microarray Analysis of the Gene Expression Profile of Swine Muscle
Chul Wook Kim, Kyu Tae Chang, Yeon Hee Hong, Won Yong Jung, Eun Jung Kwon, Kwang Keun Cho, Ki Hwa Chung, Byeong Woo Kim, Jung Gyu Lee, Jung Sou Yeo, Yang Su Kang, Young Kuk Joo
Abstract
By screening specific genes related to the muscle growth of swine using cDNA microarray technology, a total of 5 novel genes (GF (growth factor) I, II, III, IV and V) were identified. Results of southern blotting to investigate the number of copies of these genes in the genome of swine indicated that GF I, GF III, and GF V existed as one copy and GF II, and GF IV existed as more than two copies. It was suggested that there are many isoforms of these genes in the genome of swine. Also, results of northern blotting to investigate whether these genes were expressed in grown muscle, using GF I, III, and V indicated that all the genes were much more expressed in the muscle of swine with body weight of 90 kg. Expression patterns of these genes in other organs, namely muscle and propagation and fat tissues, were investigated by extracting RNA from the tissues. These genes were not expressed in the propagation and fat tissues, but were expressed in the muscle tissue. To determine the mechanism of muscle growth, further studies should be preceded using the 3 specific genes related to muscle growth, that is GF I, III, and V.
Keywords: Muscle Genes; Swine; Expression Profiles; cDNA Microarray


ABOUT
SPECIALTIES
BROWSE ARTICLES
FOR AUTHORS AND REVIEWERS
Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul
08776, Korea   TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2019 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Developed in M2community

Close layer
prev next