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Asian-Australas J Anim Sci > Volume 18(7); 2005 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2005;18(7): 933-941.
DOI: https://doi.org/10.5713/ajas.2005.933    Published online November 26, 2005.
Screening of Specific Genes Expressed in the Swine Tissues and Development of a Functional cDNA Chip
Chul Wook Kim, Kyu Tae Chang, Yeon Hee Hong, Eun Jung Kwon, Won Yong Jung, Kwang Keun Cho, Ki Hwa Chung, Byeong Woo Kim, Jung Gyu Lee, Jung Sou Yeo, Yang Su Kang, Young Kuk Joo
Abstract
To develop a functional cDNA chip, specific genes expressed in the tissues of swine Kagoshima Berkshire were screened. A total of 4,434 ESTs were obtained by constructing a cDNA library from total RNA isolated from the muscle and fat tissues, affirming their functions by investigating similarity of nucleotide sequences with the database at the NCBI. Among them, 1,230 ESTs were confirmed as novel genes, which, to date, have not been identified. Attaching the genes to a cDNA microarray slide revealed expression patterns of genes in muscle and fat according to the growth stages of swine. As specific genes expressed in the muscle tissues of swine with body weight of 30 kg, 60 genes including actin, myosin, tropomysin, transfer RNA-trp synthetase, Kel-like protein 23, KIAA0182 and COI, Foocen-m, etc were obtained. In addition, 18 novel genes were obtained. As specific genes expressed in fat tissues of swine with body weight of 30 kg, 47 genes including annexin II, Collagen, Fibronectin, Pleckstrin homology domain, serine protease, etc were obtained. 21 novel genes were also obtained. The genes specifically expressed in the muscle and fat tissues of swine affect contraction and relaxation of the muscle and the fat. However, studies on the expression mechanisms of the genes are insufficient. To reveal species of structural genes in swine muscle and fat tissue, interrelation studies in expression and function of genes by using the cDNA chip should be conducted.
Keywords: Swine Tissues; cDNA Chip; EST; Microarray


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