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Animal Products
Asian-Australasian Journal of Animal Sciences 2005;18(5): 734-740.
DOI: https://doi.org/10.5713/ajas.2005.734    Published online November 25, 2005.
Polymorphism of the Promoter Region of Hsp70 Gene and Its Relationship with the Expression of HSP70mRNA, HSF1mRNA, Bcl-2mrna and Bax-AMrna in Lymphocytes in Peripheral Blood of Heat Shocked Dairy Cows
Yafei Cai, Qinghua Liu, Guangdong Xing, Lei Zhou, Yuanyuan Yang, Lijun Zhang, Jing Li, Genlin Wang
The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5`-flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-慣 at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5`-flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-慣. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-慣mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.
Keywords: Polymorphism; hsp70 Gene; Dairy Cow; HSP70mRNA; HSF1mRNA; Bcl-2mRNA/Bax-慣mRNA
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