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Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2004;17(10): 1411-1416.
DOI: https://doi.org/10.5713/ajas.2004.1411    Published online January 1, 2004.
Screening and Characterization of Lactate Dehydrogenase-producing Microorganism
Ha Guyn Sung, Jae Heung Lee, Hyung Tai Shin
Abstract
The objective of this work was to isolate a microorganism, able to produce high lactate dehydrogenase (LDH) activity, for use as a microbial feed additive. The LDH is an important enzyme for lactate conversion in the rumen, thereby possibly overcoming lactic acidosis owing to sudden increases of cereal in the diets of ruminants. In the present study, various bacterial strains were screened from a variety of environments. Among the isolated microorganisms, strain FFy 111-1 isolated from a Korean traditional fermented vegetable food called Kimchi showed the highest enzyme activity, along with retaining strong enzyme activity even in rumen fluid in vitro. Based on morphological and biochemical characteristics as well as compositions of cellular fatty acids plus API analyses, this strain was identified as Lactobacillus sp. The optimum temperature and pH for growth were found to be 30 C and pH 6.5, respectively. A maximum cell growth of 2.2 at A650 together with LDH activity of 2.08 U per mL was achieved after 24 h of incubation. Initial characterization of FFy 111-1 suggested that it could be a potential candidate for use as a direct-fed microbial in the ruminant animals.
Keywords: Lactobacillus; Lactate Dehydrogenase; Direct-fed Microbials


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