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Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2004;17(10): 1338-1343.
DOI: https://doi.org/10.5713/ajas.2004.1338    Published online January 1, 2004.
Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line
X. M. Zhou, Y. H. Ma, W. J. Guan, D. M. Zhao
The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was successfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.
Keywords: Debao Pony; Ear Marginal Tissue; Fibroblast Cell Line; Primary Explant Technique; Collagenase Technique

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