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Asian-Australas J Anim Sci > Volume 16(5); 2003 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2003;16(5): 732-737.
DOI: https://doi.org/10.5713/ajas.2003.732    Published online January 1, 2003.
Anti-idiotypic Antibodies against Bovine Growth Hormone
N. K. Verma, R. Sodhi, Y. S. Rajput
Anti-antibodies against three mouse monoclonal antibodies viz. IIB5D6, VIA6E8 and VIC1F9 (specific to bovine growth hormone) in rabbits have been generated and characterized. Ammonium sulfate fractionated and affinity-purified monoclonal antibodies were used for producing anti-antibodies. The generated anti-antibodies were against common as well as uncommon antigenic determinants present in mouse monoclonal antibodies. The raised anti-antibodies replaced [ I125 ]bGH bound to goat liver microsomes indicating production of anti-idiotypic antibodies against bovine growth hormone. These antibodies can have profound implications in vivo in lactating bovines for enhancing milk yield.
Keywords: Anti-idiotypic Antibodies; Bovine Growth Hormone; Bovine Somatotropin; Receptor Assay; Antibody Purification
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