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Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2003;16(4): 549-554.
DOI: https://doi.org/10.5713/ajas.2003.549    Published online January 1, 2003.
Determination of Intestinal Disappearance of Lysine and Methionine Derived from Ruminally Protected Lysine and Methionine in Holstein Heifers
K. Watanabe, H. Sato, T. Kobayashi, K. Katoh, Y. Obara
In order to estimate the availability of ruminally-protected Lys (RPLys) and Met (RPMet), quantitative changes of free Lys and Met in the total abomasal outflow and feces were determined in three Holstein heifers with a T-shaped duodenal cannula. RPLys was prepared by coating Lys with fat and RPMet with a pH-sensitive polymer. RPLys and RPMet containing 30 g of each amino acid were supplied together to heifers, and total abomasal outflow was collected hourly from the T-shaped duodenal cannula for a 48 h period. Collected abomasal outflows were fractionated into liquid and solid phases to measure free Lys and Met concentration. At 2 wk after total abomasal outflow sampling, heifers were supplied RPLys and RPMet together again, and total feces were collected every 12 h for a 3 d period to estimate excreted RPLys and RPMet in feces. The amounts of Lys and Met recovered from the liquid fractions of abomasal outflow were estimated to be 23.9 8.3% and 68.6 3.6% (p=0.008) of RPLys and RPMet ingested, respectively. Final intestinal disappearances of Lys and Met were estimated to be 49.5 2.6% and 78.2% 6.5% (p=0.015) of ingested RPLys and RPMet, respectively.
Keywords: Ruminally Protected Lysine; Ruminally Protected Met; Heifer; Intestinal Disappearance
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