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Ruminant Nutrition and Forage Utilization
Asian-Australasian Journal of Animal Sciences 2003;16(2): 217-221.
DOI: https://doi.org/10.5713/ajas.2003.217    Published online February 1, 2003.
Status of Antioxidant Enzymes in Normal Cycling and α-Tocopherol Supplemented Anestrus Buffalo Heifers (Bubalus bubalis)
R. S. Kahlon, Rajvir Singh
The present investigation was undertaken to study status of erythrocytic antioxidant enzymes in normal cycling and ??tocopherol supplemented anestrus buffalo heifers. The pre-supplementation erythrocytic activities of superoxide dismutase (U/mg Hb), glutathione peroxidase (U/mg Hb) and glucose-6-phosphate dehydrogenase (U/g Hb) upregulated significantly (p<0.05) in anestrus heifers (10.08??.09, 14.09??.54, 9.25??.29) when compared to normal cycling ones (6.93??.04, 11.61??.19, 5.58??.26). The oral supplementation of ??tocopherol @ 3,000 mg per week per animal in anestrus heifers declined erythrocytic superoxide dismutase and glucose-6-phosphate dehydrogenase activities significantly (p<0.01) but led to non-significant increase in erythrocytic glutathione peroxidase activity. Results indicated that supplementation of ??tocopherol to anestrus buffalo heifers mitigated the effects of oxidative stress to improve their antioxidant status.
Keywords: Antioxidant Enzyme; a-Tocopherol; Anestrus; Buffalo Heifers

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