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Asian-Australas J Anim Sci > Volume 15(8); 2002 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2002;15(8): 1204-1209.
DOI: https://doi.org/10.5713/ajas.2002.1204    Published online January 1, 2002.
Effect of Heating on Polymerization of Pig Skin Collagen Using Microbial Transglutaminase
Yuny Erwanto, Michio Muguruma, Satoshi Kawahara, Takahiko Tsutsumi, Kazunori Katayama, Kiyoshi Yamauchi, Toshiro Morishita, Yasuko Kai, Shohei Watanabe
Abstract
Polymerization of heated or unheated pig skin collagen using microbial transglutaminase (MTGase) was investigated. Pig skin collagen samples were heated or left unheated, then enzymatically polymerized with MTGase. SDS-PAGE was conducted to confirm the intermolecular polymer and the results showed similar bands between samples without MTGase and unheated samples with MTGase. The polymerized product of pig skin collagen was not formed in unheated samples, even when MTGase was added during incubation. Different results were obtained from samples heated at 80 C and 100 C for 2 min, whereas the SDS-PAGE pattern indicated that a polymer band was generated in both cases. The heat treatment successfully modified the native structure of collagen and also made collagen more reactable in the MTGase polymerization system. Scanning Electron Microscope (SEM) investigation of pig skin collagen showed a biopolymer structure through intermolecular collagen crosslinking, while there were no intermolecular crosslinks in samples not treated with MTGase. There were no significant differences in fibril diameter between treated samples and controls. These results suggest that heat treatment of native pig skin collagen enhanced the polymerization capability of MTGase.
Keywords: Pig Skin Collagen; Microbial Transglutaminase; Biopolymer; Heat Treatment


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