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Asian-Australas J Anim Sci > Volume 15(8); 2002 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2002;15(8): 1091-1097.
DOI: https://doi.org/10.5713/ajas.2002.1091    Published online January 1, 2002.
Role of Diacyl Glycerol (DAG) in Caprine Sperm Acrosomal Exocytosis Induced by Progesterone
P. R. Somanath, K. K. Gandhi
Abstract
Capacitated goat spermatozoa generated diacyl glycerol (DAG) when suspended in Krebs Ringer bicarbonate medium and induced by progesterone or Ca2+ ionophore A23187. We have added Sn-1-oleoyl-2-acetyl glycerol externally, to study the effect of DAG in goat sperm acrosomal exocytosis. Addition of neomycin abolished the DAG generating capacity of progesterone in a dose dependent manner, suggesting the involvement of a phosphoinositidase C activated phospholipase C system in the process. The level of increase in phosphatidic acid was considerably low and was produced well after the DAG generation thereby suggesting the involvement of a DAG kinase which phosphorylates DAG to produce PA. The inhibition of progesterone mediated effect by inhibitors of GABAA/Cl channel and Ca2+ channels further supports the evidence that the events of binding of agonist to the receptor(s), opening of Ca2+ channels and the activation of phospholipase C are reconciled to perform the function of acrosome reaction in capacitated goat spermatozoa.
Keywords: Goat; Spermatozoa; Acrosome Reaction; Diacyl Glycerol; Phospholipase C
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