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Asian-Australas J Anim Sci > Volume 15(5); 2002 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2002;15(5): 615-621.
DOI: https://doi.org/10.5713/ajas.2002.615    Published online January 1, 2002.
Effect of Mitogens, Supplement of -mercaptoethanol and Fetal Bovine Serum Supplementation in Whole Blood Culture Medium for Bovine Chromosome Analysis
M. Takagi, M. Tsuno, M. Ohtani, T. J. Acosta
Abstract
We investigated the effects of the mitogen supplements of 3 types, pokeweed mitogen (PWM), phytohemagglutinin (PHA) and concanavalin A (ConA), to a whole blood culture system on the number of metaphase spreads obtained in perinatal bovine chromosome analysis. In addition, the supplementation of -mercaptoethanol ( -ME) and FBS was examined in such system. Significant differences (p<0.05) were seen in the number of metaphase spreads with PHA stimulation compared to both PWM and ConA stimulation. When examined the effects of -ME supplementation, the number of metaphase spreads was significantly (p<0.05) increased at 30 M -ME compared to control. When evaluated FBS supplementation during PWM stimulation, no significant effect of the supplementation was found. Finally, the effects of the cortisol concentration (10-20, 20-30 and >30 ng/ml) of the blood samples were examined. There was no significant effect of cortisol concentration (p>0.05) among these 3 cortisol concentration groups. The mean percentages of normal metaphase plates (2n=60) from each calf 1) with -ME, 2) without -ME and 3) with FBS stimulated with PWM were not significantly different (p>0.05). In conclusion, these findings may be useful in cytogenetic screening programs for not only perinatal calves but also for mature cattle.
Keywords: Bovine; Chromosome Analysis; #NAME?; Mitogens; Metaphase Plates
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