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Asian-Australas J Anim Sci > Volume 14(12); 2001 > Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2001;14(12): 1675-1677.
DOI: https://doi.org/10.5713/ajas.2001.1675    Published online December 1, 2001.
Effect of the Presence of Corpus Luteum on Oocyte Recovery and Subsequent in vitro Maturation and Fertilization in Buffaloes
Sajjan Singh, O. P. Dhanda, R. K. Malik
Abstract
The effect of the presence or absence of corpus luteum in the ovaries of slaughtered buffaloes was studied for the oocytes recovery and their subsequent maturation and fertilization in vitro. On an average, 0.41 and 0.67 oocytes per ovary were recovered from ovaries with and without corpus luteum, respectively. Immature oocytes were matured in TCM-199 medium. Significant difference was observed in maturation rate between good (74%) and fair (37%) oocytes. However, there was no significant difference in cleavage rate between the two types. The results of this study show that although the presence of corpus luteum in the ovary at the time of recovery significantly affected availability of total oocytes and in-vitro maturation, but fertilization and cleavage remained unaffected under in vitro conditions.
Keywords: Buffalo; Ovary; Corpus Luteum; Oocytes Recovery; In vitro Maturation; Fertilzation


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