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Asian-Australas J Anim Sci > Volume 14(12); 2001 > Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2001;14(12): 1670-1674.
DOI: https://doi.org/10.5713/ajas.2001.1670    Published online December 1, 2001.
Cloning and Expression of Lactate Dehydrogenase H Chain Gene in Adipose Tissues of Korean Cattle
H. H. Kim, M. B. Seol, D. H. Jeon, S. S. Sun, K. H. Kim, Y. J. Choi, M. G. Baik
Abstract
To understand molecular mechanisms that regulate deposition and release of intramuscular fat, a fasting-induced clone was identified by differential screening from cDNA library of adipose tissues of Korean cattle. The clone had a total length of 1,319 nucleotides coding for 334 amino acids. It was identified as one encoding L-lactate dehydrogenase H chain (LDH-B). Comparison of the deduced amino acid sequences of bovine LDH-B with those of pig, human, rat, and mouse showed 98%, 98%, 97%, and 96% identity, respectively. Food deprivation for 48 h increased mRNA levels of LDH-B gene in adipose tissues of Korean cattle compared to fed- and 6 h refed- tissues. The expression of obese mRNA was examined for individual adipose tissue from several fat depots. Fasting induced expression of LDH-B gene in subcutaneous adipose tissues, but it did not affect expression levels in abdominal, perirenal and intramuscular tissues. Results demonstrate that induction of LDH-B gene during fasting may represent a metabolic shift from anaerobic state to aerobic predominance in fasted adipose tissues and that its responses to fasting are different among several adipose tissues.
Keywords: Korean Cattle; Lactate Dehydrogenase B Gene; Adipose Tissues; Fasting


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