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Improvement of In Vitro Development of Bovine Embryos in a Medium Containing Selenium |
J. H. Lee, J. H. Park, K. M. Choi, K. S. Im, D. I. Jin |
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Abstract |
The objective of this study was to investigate the effect of selenium (Se) on in vitro development of bovine embryos. In CR1BSA, FBS-free medium, the bovine embryos could not proceed past the developmental block more efficiently to morula stage than in chemically undefined media. Addition of glutathione precursor, cysteine, with 棺- mercaptoethanol did not improve the development in chemically defined medium and neither did glutathione alone. Exogenous selenium improved the embryonic development to the morula and blastocyst stages at 6 days post-insemination (dpi) significantly (67.1% vs 57.5%, p<0.05), and blastocyst stage at 8 dpi (30.1% vs 20.5%, p>0.05). These improvements might be induced by elevated glutathione peroxidase activity due to addition of Se, and a possible mechanism of selenium to elevate the activity of glutathione peroxidase is discussed. |
Keywords:
Bovine; Embryos; Cysteine; Glutathione; Selenium; In Vitro Development |
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