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Asian-Australas J Anim Sci > Volume 14(2); 2001 > Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2001;14(2): 170-173.
DOI: https://doi.org/10.5713/ajas.2001.170    Published online February 1, 2001.
Improvement of In Vitro Development of Bovine Embryos in a Medium Containing Selenium
J. H. Lee, J. H. Park, K. M. Choi, K. S. Im, D. I. Jin
Abstract
The objective of this study was to investigate the effect of selenium (Se) on in vitro development of bovine embryos. In CR1BSA, FBS-free medium, the bovine embryos could not proceed past the developmental block more efficiently to morula stage than in chemically undefined media. Addition of glutathione precursor, cysteine, with 棺- mercaptoethanol did not improve the development in chemically defined medium and neither did glutathione alone. Exogenous selenium improved the embryonic development to the morula and blastocyst stages at 6 days post-insemination (dpi) significantly (67.1% vs 57.5%, p<0.05), and blastocyst stage at 8 dpi (30.1% vs 20.5%, p>0.05). These improvements might be induced by elevated glutathione peroxidase activity due to addition of Se, and a possible mechanism of selenium to elevate the activity of glutathione peroxidase is discussed.
Keywords: Bovine; Embryos; Cysteine; Glutathione; Selenium; In Vitro Development
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