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Asian-Australas J Anim Sci > Volume 14(1); 2001 > Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2001;14(1): 13-16.
DOI: https://doi.org/10.5713/ajas.2001.13    Published online January 1, 2001.
Cloning and Expression of β1-Adrenergic Receptor Genes in Adipose Tissues from Korean Native Cattle (Hanwoo)
S. H. Ha, M. I. Chung, M. G. Baik, Y. J. Choi
Abstract
Bovine 횩1-adrenergic receptor (AR) cDNA was cloned using degenerative primers. Bovine 횩1-AR coded for 467 amino acids and the comparison of the deduced amino acid sequence with that of sheep showed 93.4% identity. Northern blot analysis indicated that transcript size for the bovine 횩1-AR was 3.6 kb in the adipose tissue. The expression level of three 횩-ARs (1, 2, and 3) in bovine abdominal, subcutaneous, and perirenal adipose tissues were examined using reverse transcription-polymerase chain reaction (RT-PCR), and the levels of 횩1- and 횩3-AR mRNA were found to be lower in the subcutaneous adipose tissue than in the abdominal and perirenal adipose tissues. These results suggest that the expression of 횩-ARs mRNA are differentially regulated among the adipose tissues.
Keywords: 횩1-Adrenergic Receptor cDNA; Cloning; Expression; Adipose Tissue


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