• Home
  • E-Submission
  • Sitemap
  • Contact Us
Asian-Australas J Anim Sci. Search

CLOSE


Go to Top Go to Bottom
Asian-Australas J Anim Sci > Volume 9(6); 1996 > Article
Swine Nutrition and Feed Technology
Asian-Australasian Journal of Animal Sciences 1996;9(6): 671-678.
DOI: https://doi.org/10.5713/ajas.1996.671    Published online December 1, 1996.
Hemagglutination and colony hybridization for the identification of enterotoxigenic Escherichia coli isolated from healthy pig
S. H. Choi, M. J. Oh, C. Sung
Abstract
Erythrocytes from three different animal species were used to determine mannose-sensitive hemagglutination(MSHA) and mannose-resistant hemagglutination(MRHA) of 755 isolates obtained from rectal swabs of healthy pig. In addition, colony hybridization using digoxigenin-dUTP labeled polynucleotide probes was performed for the detection of heat-stable and heat-lavile enterotoxin genes carried by MRHA positive isolates. Of 755 strains, 9, 4 and 28 strains gave a positive MRHA with bovine, equine and pig erythrocytes, respectively. Of these isolates, 28(3.7%) were characterized for positive MRHA by at least one blood. Seven isolates gave a positive MRHA with two kinds of blood. Three gave a positive MRHA with three kinds of blood. Twenty-eight strains, while positive in MRHA, yielded negative signals in the colony hybridization assay for the detection of heat-stable(STaI and STAII) and geat-labile(LT) enterotoxin genes in E. coli.
Keywords: MSHA; MRHA; Colony Hybridization; Enterotoxigenic E. coli
TOOLS
Share :
Facebook Twitter Linked In Google+
METRICS Graph View
  • 0 Crossref
  •  0 Scopus
  • 1,675 View
  • 3 Download


ABOUT
SPECIALTIES
BROWSE ARTICLES
FOR AUTHORS AND REVIEWERS
Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul
08776, Korea   TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2019 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Developed in M2community

Close layer
prev next