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Swine Nutrition and Feed Technology
Asian-Australasian Journal of Animal Sciences 1996;9(6): 671-678.
DOI: https://doi.org/10.5713/ajas.1996.671    Published online December 1, 1996.
Hemagglutination and colony hybridization for the identification of enterotoxigenic Escherichia coli isolated from healthy pig
S. H. Choi, M. J. Oh, C. Sung
Abstract
Erythrocytes from three different animal species were used to determine mannose-sensitive hemagglutination(MSHA) and mannose-resistant hemagglutination(MRHA) of 755 isolates obtained from rectal swabs of healthy pig. In addition, colony hybridization using digoxigenin-dUTP labeled polynucleotide probes was performed for the detection of heat-stable and heat-lavile enterotoxin genes carried by MRHA positive isolates. Of 755 strains, 9, 4 and 28 strains gave a positive MRHA with bovine, equine and pig erythrocytes, respectively. Of these isolates, 28(3.7%) were characterized for positive MRHA by at least one blood. Seven isolates gave a positive MRHA with two kinds of blood. Three gave a positive MRHA with three kinds of blood. Twenty-eight strains, while positive in MRHA, yielded negative signals in the colony hybridization assay for the detection of heat-stable(STaI and STAII) and geat-labile(LT) enterotoxin genes in E. coli.
Keywords: MSHA; MRHA; Colony Hybridization; Enterotoxigenic E. coli
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