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Asian-Australas J Anim Sci > Volume 8(5); 1995 > Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 1995;8(5): 449-454.
DOI: https://doi.org/10.5713/ajas.1995.449    Published online October 1, 1995.
Early screening of gene expression of SV40 driven lacZ introduced into bovine embryos
A. Nakamura, J. Okumura, T. Muramatsu
Abstract
The present study was conducted to assess gene expression of bacterial lacZ driven by the SV40 promoter at early developmental stages of bovine embryos. The lacZ gene was linearized with BamH` digestion, and introduced into the pronucleus by microinjection at 20 hrs after the commencement of in vitro fertilization. Intact bovine blastocysts were not stained with X-Gal, suggesting that threre is no endogenous beta-galactosidase activity in these blastocysts. In contrast, the bacterial beta-galactosidase activity, albeit at a low rate, i.e. 2.1% of the total fertilized oocytes injected. It was concluded, therefore, that the lacZ gene driven by the SV40 promoter could be used for an indirect screening method in which the presence of transgene is evaluated from the product of transgene expression.
Keywords: Gene Expression; SV40 Promoter; LacZ; Bovine Embryo; Microinjection
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