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Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 1994;7(4): 509-512.
DOI: https://doi.org/10.5713/ajas.1994.509    Published online December 1, 1994.
Analysis of guanine plus cytosine contents of DNA of Butyrivibrio fibrisolvens by high performance liquid chromatography (HPLC)
M. Nakatani, C. Kohda, T. Inamoto, Y. Nakai, K. Ogimoto
Abstract
High performance liquid chromatography (HPLC) analysis was applied for determination of guanine plus cytosine (G + C) contents of DNA of Butyrivibrio fibrisolvens. By values of G + C contents, a reference strain and 20 wild strains of B. fibrisolvens were classified into at least two distinct subgroups, i.e. G + C contents of 18 strains were 38-40 mol% and those of 3 strains including the reference strain were 43-45 mol%. Clear relationships were not observed between G + C contents and biological properties of 21 strains of B. Fibrisolvens.
Keywords: Butyrivibrio fibrisoluvens; G + C Contents; HPLC; Rumen Bacteria
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