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Ruminant Nutrition and Forage Utilization
Asian-Australasian Journal of Animal Sciences 1994;7(3): 373-382.
DOI: https://doi.org/10.5713/ajas.1994.373    Published online September 1, 1994.
Isolation and identification of anaerobic rumen bacterium, Actinomyces SP.40 and enzymatic properties of β-1,4-endoglucanase
H. K. Min, Y. J. Choi, J. K. Ha, K. K. Cho, Y. M. Kwon, Y. H. Chang, S. S. Lee
Abstract
A bacterial strain No. 40, which produced extracelluar ecdoglucanase, was isolated from the rumen of Korean native goats and identified to be a genus of Actinomyces sp. The optimum conditions for indoglucanase production in PY-CMC medium were initial pH of 7.0 and 4 days of cultivation at 39째C. When localization of endoglucanase activity of Actinomyces sp. was determined, 68% of the enzyme activity was found in the extracellular fraction, 11% of the activity was detected in the periplasmic space and the remaining activity was in the intracellular and cell-bound fractions. The maximal endoglucanase activity was observed at pH 5.0 and it was most stable at pH 5.0. The optimum temperature of this enzyme activity was 55째C, but enzyme activity was gradually lost at temperature above 60째C. The crude enzyme was activated by addition of 10 mM cysteine and 10 mM DTT. But it was inhibited by addition of 10mM Cu++ and Fe++. This crude enzyme could digest carboxymethycellulose (CMC), and degrade xylan, avicel, pNPG, and pNPC to a less extent.
Keywords: Actinomyces sp. Endoglucanase; Korean Native Goat
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