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Ruminant Nutrition and Forage Utilization
Asian-Australasian Journal of Animal Sciences 1994;7(2): 223-229.
DOI: https://doi.org/10.5713/ajas.1994.223    Published online June 1, 1994.
Studies on methionine metabolism in the rumen bacteria of goats
T. Muramatsu, M. Numa, Y. Ueda, M. Furuse, J. Okumura, K. Samukawa
Abstract
The metabolic fate of methionine in rumen bacteria was studied by intraruminal administration of 15N and 1-13C labeled methionine in goats. Time course changes in isotopic abundance of amino acids in the rumen bacteria were determined with a computer-controlled gas-chromatograph mass spectrometer. The results from the transition of peak isotopic abundance in amino acids indicated that in rumen bacteria the 15N or 13C isotope in the methionine molecule was transferred rapidly to into bacteria, methionine administered intraruminally may not be retained as it is, but would be converted quickly to other metabolites in the bacteria.
Keywords: Goats; Rumen Bacteria; Methionine Metabolism; Isotope Abundance


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