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Asian-Australas J Anim Sci > Volume 6(4); 1993 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 1993;6(4): 581-589.
DOI: https://doi.org/10.5713/ajas.1993.581    Published online December 1, 1993.
Stable transformation of cultured chicken cells
J. Y. Han, Y. S. Shin, R. N. Shoffner, K. S. Guise
Abstract
A plasmid vector, RSVLTR/棺G2, containing lacZ gene under the control of the RSVLTR promoter were transfected into chicken embryo fibroblasts by three different transfection methods. Calcium phosphate, lipsome and DEAE-dextran techniques were applied for transfection of chicken cells. A histochemical assay with X-gal was used as a simple method for screening transfected cells. Plasmid RSVLTR/棺G2-was expressed proficiently in the chicken embryo fibroblast. Calcium phosphate-DNA precipitate transfection resulted in the highest efficiency for transient expression of RSVLTR/棺G2. Transfected cells formed colonies on the 9th day of incubation indicating stable transformation of the inserted plasmid.
Keywords: Plasmid Vector; Transfection; Calcium Phosphate; Liposome; DEAE-Dextran; Chicken


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