Go to Top Go to Bottom
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 1993;6(4): 581-589.
DOI: https://doi.org/10.5713/ajas.1993.581    Published online December 1, 1993.
Stable transformation of cultured chicken cells
J. Y. Han, Y. S. Shin, R. N. Shoffner, K. S. Guise
A plasmid vector, RSVLTR/棺G2, containing lacZ gene under the control of the RSVLTR promoter were transfected into chicken embryo fibroblasts by three different transfection methods. Calcium phosphate, lipsome and DEAE-dextran techniques were applied for transfection of chicken cells. A histochemical assay with X-gal was used as a simple method for screening transfected cells. Plasmid RSVLTR/棺G2-was expressed proficiently in the chicken embryo fibroblast. Calcium phosphate-DNA precipitate transfection resulted in the highest efficiency for transient expression of RSVLTR/棺G2. Transfected cells formed colonies on the 9th day of incubation indicating stable transformation of the inserted plasmid.
Keywords: Plasmid Vector; Transfection; Calcium Phosphate; Liposome; DEAE-Dextran; Chicken

Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2020 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Close layer
prev next