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Asian-Australas J Anim Sci > Volume 6(4); 1993 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 1993;6(4): 483-489.
DOI: https://doi.org/10.5713/ajas.1993.483    Published online December 1, 1993.
Modulation of insulin-stimulated DNA synthesis by cholera toxin in bovine mammary fibroblasts
I. S. Yuh, C. K. Park, J. Y. Han, L. G. Sheffield
Abstract
Bovine fibroblasts were cultured in Dulbecco`s Modified Eagle`s Medium and then treated with control, insulin (1, 1 關g/ml), cholera toxin (CT, 0.1-100 ng/ml) or CT (0.1-100 ng/ml) + 1 (1 關g/ml). cholera toxin, an activator of adenylate cyclase, significantly decreased insulin induced DNA synthesis (p<0.05). The modulation of DNA synthesis apparently involves events occurring in early stage of cell growth, at least between the first 4 and 8 hor of CT treatment. Insulin induced collagen as well as noncollagen synthesis in cell layer, however, these syntheses were reduced by addition of cholera toxin (p<0.05) but were not completely reduced. It is not clear whether the reduction of insulin-induced cell layer collagen or noncollagen proteins by CT is involved in the inhibitory effect on insulin-induced DNA synthesis. However, we could rule out the hypothesis that insulin-induced DNA synthesis is reduced by CT-induced cellular differentiation.
Keywords: Bovine Fibroblast; Cholera Toxin; Insulin; DNA Synthesis
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