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Ruminant Nutrition and Forage Utilization
Asian-Australasian Journal of Animal Sciences 1992;5(3): 465-468.
DOI: https://doi.org/10.5713/ajas.1992.465    Published online September 1, 1992.
Effect of level of feed intake on the excretion of purine derivatives and purine derivatives to creatinine ratio in the urine of sheep
Y. K. Han, H. T. Shin, J. Landis
Abstract
Urinary purine derivatives and creatinine excretion was measured in a total of 4 white Alpine sheep. They were given diets 718 to 1060 g/kg dry matter (DM) of roughage. The crude protein content of this diets was on average 93.87 5.57g in kg DM. Purine derivatives-N excretion increased linearly with incremental DM intake and was significantly correlated (n = 16) with amounts of digestible organic matter (DOM) intake: allantoin-N (mg) = 1.205 ( 0.070) 횞 DOM (g) - 136.709 ( 37.399), r = 0.9770, RSD = 22.97; uricacid-N (mg) = 1.205 ( 0.070) 횞 DOM (g) + 11.380 ( 21.881), r = 0.6306, RSD = 13.44; Hypoxanthine-N (mg) = 0.049 ( 0.014) 횞 DOM (g) - 28.640 ( 7.708), r = 0.6544, RSD = 4.73; total purine derivatives-N (mg) = 1.385 ( 0.083) 횞 DOM (g) - 90.261 ( 44.552), r = 0.9706, RSD = 27.47. Microbial protein synthesis per kg DOM was estimated of 113 g. The urinary creatinine-N excretion was on average 9.10 mg/kg live weight (LW) with a standard error of 0.12 mg creatinine-N per kg LW. The excretion of creatinine excreton was not related to feed intake. Daily creatinine excretion (mg/d) was calculated from individual LW measurements and the average creatinine excretion (mg/kg LW). It was possible to predict the daily urinary purine derivatives excretion (r = 0.9720 for allantoin, r = 0.9886 for total purine derivatives) from the ratio of purine derivatives (mg/100 ml) and creatinine (mg/100 ml) in the urine and the daily creatinine excretion.
Keywords: Purine Derivatives; Creatinine; Microbial Protein; Digestible Organic Matter


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