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Asian-Australas J Anim Sci > Volume 2(4); 1989 > Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 1989;2(4): 595-598.
DOI: https://doi.org/10.5713/ajas.1989.595    Published online December 1, 1989.
Co-culture of bovine embryos with cumulus cells
K. Goto, M. Koba, Y. Takuma, Y. Nakanishi, K. Ogawa
Abstract
Bovine embryos/ova obtained from in-vitro fertilization were either co-cultured on a monolayer of bovine cumulus cells or cultured in medium alone. Embryos/ova co-cultured with cumulus cells developed to 8-cell (30.9%), morula (29.8%) and blastocyst stages (26.6%) after 3-4, 5-6, and 7-8 days of culture, respectively, while embryos/ova cultured in medium alone failed to develop beyond 8-cell (0-13.3%), morula (0-1.5%) and blastocyst stages (0%). The results of this study demonstrated the beneficial effect of cumulus cells on the development of bovine embryos.
Keywords: In-Vitro Fertilization; Embryo; Bovine; Cumulus Cells; Co-Culture
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