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Asian-Australas J Anim Sci > Volume 17(5); 2004 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2004;17(5): 583-587.
DOI: https://doi.org/10.5713/ajas.2004.583    Published online January 1, 2004.
Genetic Differentiation between Sheep and Goats Based on Microsatellite DNA
W. Sun, H. Chang, Z. J. Ren, Z. P. Yang, R. Q. Geng, S. X. Lu, L. Du, K. Tsunoda
Abstract
The 7 sheep microsatellite markersOarFCB48, OarAE101, MAF33, OarFCB11, MAF70, OarFCB304 and OarFCB128, which were located on chromosomes 2, 4, 6, 9, 17 and 19, were selected to PCR in Hu sheep, Tong sheep and theidxr closely related species,the goat. They were studied with the amplifying result of 7 microsatellite sites of Hu Sheep, Tong Sheep and goats, the data of allele number and range of allele''''s size of amplifying were analyzed with ANOVA. The results showed that there were no significant differences (p<0.05) in microsatellite DNA sites among 3 populations. Concerning the conservation of microsatellites in closely related species, selecting microsatellite sites located on the chromosome where the Robertsonian fusion was caused between sheep and goat, may be used in research into genetic differentiation and evolutionary relationships between sheep and goats.
Keywords: Sheep; Goat; Microsatellite DNA; Genetic Differentiation


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