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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0761    [Accepted] Published online April 12, 2020.
Evaluation of liquid and powdered forms of polyclonal antibody preparation against Streptococcus bovis and Fusobacterium necrophorum in cattle adapted or not adapted to highly fermentable carbohydrate diets
Eduardo Cuellar Orlandi Cassiano1  , Flávio Perna Júnior1  , Tarley Araújo Barros1  , Carolina Tobias Marino2,*  , Rodrigo Dias Lauritano Pacheco3  , Fernanda Altieri Ferreira1  , Danilo Domingues Millen4  , Maurício Furlan Martins1  , Silvana Marina Piccoli Pugine5  , Mariza Pires de Melo5  , Karen Beauchemin6  , Paula Marques Meyer7  , Mário de Beni Arrigoni8  , Paulo Henrique Mazza Rodrigues1 
1Department of Animal Nutrition and Production, University of São Paulo (FMVZ-USP), Pirassununga, São Paulo 13635-900, Brazil
2Embrapa Beef Cattle, Campo Grande, Mato Grosso do Sul 79106-550, Brazil
3Mato Grosso State Agricultural and Extension Service, Varzea Grande, Mato Grosso 78115-100, Brazil
4São Paulo State University (UNESP), Dracena, São Paulo 17900-000, Brazil
5Department of Basic Sciences (ZAB), Faculty of Animal Science and Food Engineering (FZEA-USP), Pirassununga, São Paulo 13635-900, Brazil
6Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, 5403 1st Avenue South, Lethbridge, AB, Canada T1J 4B1
7Brazilian Institute of Geography and Statistics (IBGE), Pirassununga, São Paulo 13630-095, Brazil
8Department of Animal Breeding and Nutrition, São Paulo State University (FMVZ-UNESP), Botucatu, São Paulo 18618-000, Brazil
Correspondence:  Carolina Tobias Marino, Tel: +55-11992692022, Fax: +55-11992692022, Email: caroltobias@hotmail.com
Received: 1 October 2019   • Revised: 5 December 2019   • Accepted: 23 March 2020
Abstract
Objective
Feed additives that modify rumen fermentation can be used to prevent metabolic disturbances such as acidosis and optimize beef cattle production. The study evaluated the effects of liquid and powdered forms of polyclonal antibody preparation (PAP) against Streptococcus bovis and Fusobacterium necrophorum on rumen fermentation parameters in ruminally cannulated non-lactating dairy cows that were adapted or unadapted to a high concentrate diet.
Methods
A double 3 × 3 Latin square design was used with three PAP treatments (control, powdered and liquid PAP) and two adaptation protocols (adapted, unadapted; applied to the square). Adapted animals were transitioned for 2 weeks from an all-forage to an 80% concentrate diet, while unadapted animals were switched abruptly.
Results
Interactions between sampling time and adaptation were observed; 12 h after feeding, the adapted group had lower ruminal pH and greater total short chain fatty acid concentrations than the unadapted group, while the opposite was observed after 24 h. Acetate:propionate ratio, molar proportion of butyrate and ammonia nitrogen concentration were generally greater in adapted than unadapted cattle up to 36 h after feeding. Adaptation promoted 3.5 times the number of Entodinium protozoa but copy numbers of S. bovis and Fibrobacter succinogens genes in rumen fluid were not affected. However, neither liquid nor powdered forms of PAP altered rumen acidosis variables in adapted or unadapted animals.
Conclusion
Adaptation of cattle to highly fermentable carbohydrate diets promoted a more stable ruminal environment, but PAP was not effective in this study in which no animal experienced acute or sub-acute rumen acidosis.
Keywords: Acute phase protein; Feed additive; Passive immunization; Real-time PCR; Rumen fermentation
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