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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0308    [Accepted] Published online October 21, 2019.
Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices
Dimas Hand Vidya Paradhipta1,2  , Young Ho Joo1  , Hyuk Jun Lee1  , Seong Shin Lee1  , Youn Sig Kwak1  , Ouk Kyu Han3  , Dong Hyeon Kim1  , Sam Churl Kim1,* 
1Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University, Jinju 52828, Korea
2Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
3Department of Crop Science, Korea National College of Agriculture and Fisheries, Jeonju 54874, Korea
Correspondence:  Sam Churl Kim, Tel: +82-55-772-1947, Fax: +82-55-772-1949, Email: kimsc@gnu.ac.kr
Received: 11 April 2019   • Revised: 5 July 2019   • Accepted: 2 September 2019
Abstract
Objective
This study was conducted to confirm the effects of new inoculants producing-antifungal or esterase substances on rye silage and its rumen fermentation indices by comparing wild with mutated types.
Methods
Rye harvested at dough stage was ensiled into 3 L mini bucket silo (1 kg) for 90 d in triplicate following: distilled water at 20 uL/g (CON); Lactobacillus brevis 100D8 (AT) and its inactivation of antifungal genes (AT-m) at 1.2 × 105 cfu/g, respectively; and Leuconostoc holzapfelii 5H4 (FD) and its inactivation of esterase genes (FD-est) at 1.0 × 105 cfu/g, respectively. After silo opened, silage was sub-sampled for the analysis of ensiling quality and its rumen fermentation indices.
Results
Among the wild type inoculants (CON vs. AT vs. FD), FD inoculant had higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in rumen, while AT inoculant had higher (p<0.05) lactate, acetate, and lactic acid bacteria in silage. Silage pH and the potentially degradable fraction in rumen increased (p<0.05) by inactivation of antifungal activity (AT vs. AT-m), but lactate, acetate, and lactic acid bacteria of silage decreased (p<0.05). In silage, acetate increased (p<0.05) by inactivation of esterase activity (FD vs. FD-est) with decreases (p<0.05) of pH, ammonia-N, lactate, and yeast. Moreover, inactivation of esterase activity clearly decreased (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in the rumen.
Conclusion
This study concluded that FD inoculant confirmed esterase activity on rye silage harvested at dough stage, while AT inoculant could not be confirmed with antifungal activity due to the absence of mold in all silages.
Keywords: Antifungal; Esterase; Inoculant; Rye; Silage


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