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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0157    [Accepted] Published online October 21, 2019.
Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity
Xiuxue Dong1, Bing Hu1, Wenlong Wan1, Yanzhang Gong1,*, Yanping Feng1,*
College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China
Correspondence:  Yanzhang Gong, Tel: +86-27-15347112816, Fax: no, Email: poultry@mail.hzau.edu.cn
Yanping Feng,Email: fengyanping594@mail.hzau.edu.cn
Received: 26 February 2019   • Revised: 8 May 2019   • Accepted: 10 October 2019
Abstract
Objective
This study was to evaluate the effect of husbandry systems and strains on cecum microbial diversity of Jingyang chickens under the same dietary conditions.
Methods
A total of 320 laying hens (body weight [BW]: 1.70±0.15 kg, 47 weeks old) were randomly allocated to one of the four treatments: i) Silver-feathered hens in Enrichment Cages (SEC) with an individual cage (70 x 60 x 75 cm), ii) Silver-feathered hens in Free Range (SFR) with the stocking density of 1.5 chickens per ten square meters, iii) Gold-feathered hens in Enrichment Cages (GEC), iv) Gold-feathered hens in Free Range (GFR). The experiment lasted 8 weeks and the cecum fecal samples were collected for 16S rDNA high throughput sequencing at the end of experiment.
Results
(ⅰ) The core microbiota was composed of Bacteroidetes (49%~60%), Firmicutes (21%~32%) and Proteobacteria (2%~4%) at the phylum level. (ⅰⅰ) The core bacteria were Bacteroides (26%~31%), Rikenellaceae (9%~16%), Parabacteroides (2%~5%) and Lachnoclostridium (2%~6%) at the genus level. (ⅲ) The indexes of OTU, Shannon, Simpson and observed species were all higher in SFR group than in SEC group while in GEC group than in GFR group, with SFR group showing the greatest diversity of cecum microorganisms among the four groups. (ⅰv) The clustering result was consistent with the strain classification, with a similar composition of cecum bacteria in the two strains of laying hens.
Conclusion
The core microbiota was not altered by husbandry systems or strains. The free-range system increased the diversity of cecal microbes only for silver feathered hens. However, the cecum microbial composition was similar in two strain treatments under the same dietary conditions.
Keywords: Jingyang Chicken; Husbandry System; Cecum Microbiome; Diversity; Illumina MiSeq Sequencing


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