Go to Top Go to Bottom
Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0092    [Accepted] Published online May 28, 2019.
Impact of Ecklonia stolonifera extract on in vitro ruminal fermentation characteristics, methanogenesis, and microbial populations
Shin Ja Lee1  , Jin Suk Jeong2  , Nyeon Hak Shin3  , Su Kyoung Lee4  , Hyun Sang Kim5  , Jun Sik Eom5  , Sung Sill Lee1,2,* 
1Institute of Agriculture and Life Science & University-Centered Labs, Gyeongsang National University, Jinju 52828, Korea
2Division of Applied Life Science (BK21Plus) and Institute of Agriculture & Life Science (IALS), Gyeongsang National University, Jinju 52828, Korea
3Livestock Experiment Station, Gyeongsangnamdo Livestock Promotion Research Institute, Sancheong 52733, Korea
4Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea
5Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 52828, Korea
Correspondence:  Sung Sill Lee, Tel: +82-55-772-1883, Fax: +82-55-772-1889, Email: lss@gnu.ac.kr
Received: 1 February 2019   • Revised: 8 April 2019   • Accepted: 16 May 2019
Abstract
Objective
This study was conducted to evaluate the effects of <i>E. stolonifera</i> extract addition on <i>in vitro</i> ruminal fermentation characteristics, methanogenesis and microbial populations.
Methods
Cannulated Holstein cows (450 ± 30 kg) consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (09:00 and 17:00) at 2% of body weight with free access to water and mineral block were used as rumen fluid donors. <i>In vitro</i> fermentation experiment, with timothy hay as substrate, was conducted for up to 72 h, with <i>E. stolonifera</i> extract added to achieve final concentration 1, 3 and 5% on timothy hay basis.
Results
Administration of <i>E. stolonifera</i> extract to a ruminant fluid-artificial saliva mixture <i>in vitro</i> increased the total gas production. Unexpectedly, <i>E. stolonifera</i> extracts appeared to increase both methane emissions and hydrogen production, which contrasts with previous observations with brown algae extracts used under <i>in vitro</i> fermentation conditions. Interestingly, real-time polymerase chain reaction indicated that as compared with the untreated control the ciliate-associated methanogen and <i>Fibrobacter succinogenes</i> populations decreased, whereas the <i>Ruminococcus flavefaciens</i> population increased as a result of <i>E. stolonifera</i> extract supplementation.
Conclusions
<i>E. stolonifera</i> showed no detrimental effect on rumen fermentation characteristics and microbial population. Through these results <i>E. stolonifera</i> has potential as a viable feed supplement to ruminants.
Keywords: Ecklonia stolonifera Extract; In vitro Fermentation; Methane Emission; Microbial Population


Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2019 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Close layer
prev next