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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.18.0807    [Accepted] Published online March 7, 2019.
Integrated mRNA and miRNA profile expression in livers of Jinhua and Landrace pigs
Minjie Huang1  , Lixing Chen1  , Yifei Shen1  , Jiucheng Chen1  , Xiaoling Guo1  , Ningying Xu1,* 
College of Animal Science, Zhejiang University, Hangzhou 310058, China
Correspondence:  Ningying Xu, Tel: +86-571-8898-2089, Fax: +86-571-8898-2089, Email: nyxu@zju.edu.cn
Received: 26 October 2018   • Revised: 13 December 2018   • Accepted: 26 February 2019
Abstract
Objective: To explore the molecular mechanisms of fat metabolism and deposition in pigs, an experiment was conducted to identify hepatic mRNAs and miRNAs expression and determine the potential interaction of them in two phenotypically extreme pig breeds.
Methods:mRNA and miRNA profiling of liver from 70-day Jinhua (JH) and Landrace (LD) pigs were performed using RNA sequencing. Blood samples were taken to detect results of serum biochemistry. Bioinformatics analysis were applied to construct differentially expressed miRNA-mRNA network.

Results

Serum total triiodothyronine (TT3) and total thyroxine (TT4) were significantly lower in Jinhua pigs, but the content of serum total cholesterol (TCH) and low-density lipoprotein cholesterol (LDLC) were strikingly higher. A total of 467 differentially expressed genes (DEGs) and 35 differentially expressed miRNAs (DE miRNAs) were identified between JH and LD groups. Gene ontology analysis suggested that DEGs were involved in oxidation-reduction, lipid biosynthetic and lipid metabolism process. Interaction network of DEGs and DE miRNAs were constructed, according to target prediction results.
Conclusion
We generated transcriptome and miRNAome profiles of liver from Jinhua and Landrace pig breeds which represent distinguishing phenotypes of growth and metabolism. The potential miRNA-mRNA interaction networks may provide a comprehensive understanding in the mechanism of lipid metabolism. These results serve as a basis for further investigation on biological functions of miRNAs in the porcine liver.
Keywords: mRNA; miRNA; Liver; Pig; RNA-seq
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