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Asian-Australas J Anim Sci > Volume 30(8); 2017 > Article
Asian-Australasian Journal of Animal Sciences 2017;30(8): 1124-1134. doi: https://doi.org/10.5713/ajas.16.0677
Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement
A. A. Omede1,2 , M. M. Bhuiyan1, A. F. lslam1, P. A. Iji1,*
1School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia
2Department of Animal Production, Kogi State University, PMB 1008, Anyigba, 272102, Kogi State, Nigeria
* Corresponding Author: P. A. Iji ,Tel: +61-2-6773-2082, Fax: +61-2-6773-3922, Email: piji@une.edu.au
Received: September 7, 2016;  Revised: October 7, 2016.  Accepted: January 25, 2017.  Published online January 26, 2017.

ABSTRACT
Objective:
This study explored the physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed (IOF) supplement.
Method:
Amniotic fluid was collected from broiler breeders (Ross 308, 51 weeks and Cobb 500, 35 weeks) on day 17 after incubation. A mixture of high-quality soy protein supplement – Hamlet Protein AviStart (HPA) was serially diluted in MilliQ water to obtain solutions ranging from 150 to 9.375 mg/mL. The mixtures were heat-treated (0, 30, 60 minutes) in a waterbath (80°C) and then centrifuged to obtain supernatants. The amniotic fluid and HPA supernatants were analysed for their physico-chemical properties.
Results:
Only viscosity and K+ were significantly (p<0.05) different in both strains. Of all essential amino acids, leucine and lysine were in the highest concentration in both strains. The osmolality, viscosity and pCO2 of the supernatants decreased (p<0.05) with decreasing HPA concentration. Heat treatment significantly (p<0.05) affected osmolality, pH, and pCO2, of the supernatants. The interactions between HPA concentration and heat treatment were significant with regards to osmolality (p<0.01), pH (p<0.01), pCO2 (p<0.05), glucose (p<0.05), lactate (p<0.01) and acid-base status (p<0.01) of HPA solutions. The Ca2+, K+, glucose, and lactate increased with increasing concentration of HPA solution. The protein content of HPA solutions decreased (p<0.05) with reduced HPA solution concentrations. The supernatant from 150 mg/mL HPA solution was richest in glutamic acid, aspartic acid, arginine and lysine. Amino acids concentrations were reduced (p<0.05) with each serial dilution but increased with longer heating.
Conclusion:
The values obtained in the primary solution (highest concentration) are close to the profiles of high-protein ingredients. This supplement, as a solution, hence, may be suitable for use as an IOF supplement and should be tested for this potential.
Keywords: Amino Acids; Amniotic Fluids; In ovo; Physico-chemical Properties; Protein
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