Anim Biosci > Volume 36(2); 2023 Special Issue > Article |
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Targeted gene | Knockout or Knockin | Technical platforms | Phenotype | Reference |
---|---|---|---|---|
Immunoglobulin | Knockout | Homologous recombination | No antibody response on immunization | [6] |
OV | Knockout | TALEN | Chicken ovalbumin gene knockout | [5] |
DDX4 | Knockin | TALEN | Germ cell-specific reporter gene expression | [33] |
G0S2 | Knockout | CRISPR-Cas9 | Reduction of fat deposition | [7] |
MSTN | Knockout | CRISPR-Cas9 | Increased muscle growth & reduction of fat deposition |
[8] (chicken) [16] (quail) |
tva, tvc, tvj | Knockout | CRISPR-Cas9 | ALV-resistance | [17] |
OVM | Knockout | CRISPR-Cas9 | No deposition of ovomucoid in egg white | [30] |
DMRT1 | Knockout | CRISPR-Cas9 | DMRT1 function as sex determination | [31] |
OV | Knockin | CRISPR-Cas9 | Deposition of hIFN-β in egg white | [21] |
Z chr-targeted | Knockin | CRISPR-Cas9 | Male-specific GFP expression | [32] |
OV, ovalbumin; TALEN, transcription activator-like effector nucleases; DDX4, DEAD-box helicase 4; G0S2, G0/G1 switch gene 2; CRISPR-Cas9, clustered regularly interspaced short palindromic repeat-CRISPR associated protein 9; MSTN, myostatin; OVM, ovomucin; DMRT1, doublesex and mab-3 related transcription factor 1; hIFN-β, human interferon β; GFP, green fluorescent protein.