Anim Biosci > Volume 34(6); 2021 > Article |
|
DM, dry matter; FW, fresh weight; CP, crude protein; WSC, water soluble carbohydrate; NDF, neutral detergent fibre; ADF, acid detergent fibre; ADL, acid detergent lignin; BC, buffering capacity; mEq, milligram equivalent; FC, fermentation coefficient; cfu, colony-forming units; LAB, lactic acid bacteria; EB, enterobacteria; AB, aerobic bacteria.
Items | Treatments1) | Ensiling days | Mean | SEM | p-value2) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|||||||||||||
3 | 6 | 9 | 15 | 30 | 60 | D | F | F-L | F-Q | D×F | ||||
pH | Control | 5.44Aa | 5.23Aab | 4.65b | 4.72ABb | 4.68ABb | 4.78ABb | 4.92A | 0.052 | 0.007 | <0.001 | <0.001 | 0.085 | <0.001 |
0.2% FA | 5.06Ba | 5.03Aa | 4.49b | 4.30Cb | 4.19Bb | 4.28Bb | 4.56B | |||||||
0.4% FA | 4.32Cc | 4.64Bbc | 4.52bc | 4.80Aabc | 5.18Aab | 5.43Aa | 4.82A | |||||||
0.6% FA | 4.03Ca | 4.24Ca | 4.13a | 4.33BCa | 4.28ABa | 4.30Ba | 4.27C | |||||||
Lactic acid (% DM) | Control | 1.23Ac | 1.92Ac | 2.51Aabc | 3.67Aab | 3.92Aa | 2.28Bbc | 2.59A | 0.210 | <0.001 | <0.001 | <0.001 | 0.020 | <0.001 |
0.2% FA | 0.49Bc | 1.18Bbc | 1.81ABbc | 3.49Aab | 5.78Aa | 5.54Aa | 3.05A | |||||||
0.4% FA | 0.05Bc | 0.11Cc | 0.27Bbc | 0.63Bab | 0.81Bab | 1.14Ca | 0.50B | |||||||
0.6% FA | 0.04Bc | 0.07Cc | 0.16Bbc | 0.11Bbc | 0.42Bab | 0.71Ca | 0.25B | |||||||
Acetic acid (% DM) | Control | 0.69Ae | 0.83Ade | 1.01Ad | 1.30Ac | 2.24Ab | 2.58Aa | 1.44A | 0.072 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
0.2% FA | 0.30Bc | 0.38Bbc | 0.40Babc | 0.52Babc | 0.60Bab | 0.63Ba | 0.47B | |||||||
0.4% FA | 0.23Bd | 0.19Cd | 0.25Ccd | 0.37Bbc | 0.46BCb | 0.74Ba | 0.37C | |||||||
0.6% FA | 0.23Bc | 0.20Cc | 0.20Cc | 0.25Bbc | 0.44Ca | 0.38Cab | 0.28D | |||||||
Butyric acid (% DM) | Control | NDb | NDb | NDb | 0.04b | 0.17Aa | 0.21Aa | 0.07A | 0.010 | <0.001 | <0.001 | <0.001 | 0.028 | <0.001 |
0.2% FA | ND | ND | ND | ND | NDB | NDB | 0.00B | |||||||
0.4% FA | NDb | NDb | 0.03b | 0.04b | 0.22Aa | 0.27Aa | 0.09A | |||||||
0.6% FA | ND | ND | ND | ND | NDB | NDB | 0.00B | |||||||
LA/AA | Control | 1.77Aab | 2.31Aab | 2.46ABa | 2.84ABa | 1.75Bab | 0.88Cc | 2.00B | 0.032 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
0.2% FA | 1.62Ac | 3.32Abc | 4.43Aabc | 7.44Aabc | 9.57Aa | 8.80Aab | 5.86A | |||||||
0.4% FA | 0.20B | 0.57B | 1.08B | 2.01AB | 1.72B | 1.55BC | 1.19BC | |||||||
0.6% FA | 0.17Bb | 0.38Bb | 0.80Bb | 0.45Bb | 1.02Bab | 1.90Ba | 0.78C |
SEM, standard error of means; DM, dry matter; LA/AA, ratio of lactic acid to acetic acid; ND, not detected.
1) Control, no additive; 0.2% FA, 0.2% formic acid; 0.4% FA, 0.4% formic acid; 0.6% FA, 0.6% formic acid;
Items (log10 cfu/g FW) | Treatments1) | Ensiling days | Mean | SEM | p-value2) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|||||||||||||
3 | 6 | 9 | 15 | 30 | 60 | D | F | F-L | F-Q | D×F | ||||
LAB | Control | 5.64Aabc | 6.23Aa | 5.91Aab | 5.53Bbc | 5.20Bcd | 4.75Bd | 5.54B | 0.158 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
0.2% FA | 4.15Bd | 5.68Bc | 6.09Abc | 6.72Aa | 7.05Aa | 6.13Ab | 5.97A | |||||||
0.4% FA | 3.38Cc | 3.43Cc | 3.69Bbc | 4.25Cb | 5.10Ba | 4.93Ba | 4.13C | |||||||
0.6% FA | 2.53Dd | 2.74Dcd | 3.13Bbc | 3.33Db | 3.98Ca | 2.90Cbcd | 3.10D | |||||||
EB | Control | 8.43Aa | 7.70Aab | 7.11Ab | 6.86Ab | 3.89Cc | 3.94Cc | 6.32A | 0.255 | <0.001 | <0.001 | <0.001 | 0.375 | <0.001 |
0.2% FA | 7.92Aa | 7.24Ab | 6.81Ac | 6.59Ac | NDDd | NDDd | 4.76B | |||||||
0.4% FA | 5.95Bb | 6.10Bab | 6.35Bab | 6.71Aab | 6.99Aa | 6.53Aab | 6.44A | |||||||
0.6% FA | 3.61Cc | 3.83Cbc | 3.99Cb | 5.62Ba | 5.69Ba | 5.88Ba | 4.77B | |||||||
Yeasts | Control | 5.25Aa | 4.86Aab | 4.82Aab | 4.75Aab | 4.23Ab | 4.59Aab | 4.75A | 0.063 | <0.001 | <0.001 | <0.001 | 0.468 | 0.001 |
0.2% FA | 3.94Acd | 4.52Aab | 4.76Aa | 4.02Abcd | 4.38Babc | 3.81Bd | 4.24BC | |||||||
0.4% FA | 3.89Bc | 3.91Bc | 3.94Bc | 4.56Abc | 5.39Aa | 5.07Aab | 4.46AB | |||||||
0.6% FA | 3.78Bb | 3.82Bb | 3.82Bb | 4.09Aab | 4.23Ba | 3.91Bb | 3.94C | |||||||
Moulds | Control | 4.92A | 4.80A | 4.31A | 3.73A | 3.38A | 3.45A | 4.10A | 0.211 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
0.2% FA | 4.05Ba | 4.12ABa | 3.89Aa | 2.56Bb | NDBc | NDBc | 2.44C | |||||||
0.4% FA | 2.48Cc | 3.55Bab | 4.06Aab | 4.32Aa | 4.42Aa | 3.29Abc | 3.69B | |||||||
0.6% FA | NDD | NDC | NDB | NDC | NDB | NDB | 0.00D | |||||||
AB | Control | 7.03Aa | 6.41Ab | NDBc | NDc | NDc | NDc | 2.24A | 0.269 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
0.2% FA | 4.57Cb | 5.02Ba | NDBc | NDc | NDc | NDc | 1.60B | |||||||
0.4% FA | 5.31BCa | 4.50Cb | 4.64Ab | NDc | NDc | NDc | 2.41A | |||||||
0.6% FA | 3.68Da | NDDb | NDBb | NDb | NDb | NDb | 0.61C |
cfu, colony-forming units; FW, fresh weight; SEM, standard error of means; LAB, lactic acid bacteria; EB, enterobacteria; ND, not detected; AB, aerobic bacteria.
1) Control, no additive; 0.2% FA, 0.2% formic acid; 0.4% FA, 0.4% formic acid; 0.6% FA, 0.6% formic acid.
Items | Treatments1) | Ensiling days | Mean | SEM | p-value2) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|||||||||||||
3 | 6 | 9 | 15 | 30 | 60 | D | F | F-L | F-Q | D×F | ||||
DM (% FW) | Control | 35.2Ba | 32.7Bbc | 34.2Bab | 32.8Bbc | 32.0Bbc | 31.8Cc | 33.1C | 0.380 | <0.001 | <0.001 | <0.001 | <0.001 | 0.613 |
0.2% FA | 40.0A | 39.3A | 38.9A | 38.1A | 37.7A | 36.8A | 38.5B | |||||||
0.4% FA | 39.5Aab | 40.2Aa | 38.6Aabc | 36.9ABabc | 35.7ABbc | 34.5Bc | 37.6B | |||||||
0.6% FA | 40.7A | 40.6A | 40.8A | 41.0A | 39.2A | 38.6A | 40.1A | |||||||
DM loss (% DM) | Control | 8.90Ac | 12.8Ab | 12.4Abc | 14.3Aab | 15.4Aab | 16.8Aa | 13.4A | 0.559 | <0.001 | <0.001 | <0.001 | 0.015 | 0.018 |
0.2% FA | 6.70Ab | 8.84ABb | 10.0ABab | 10.7Aa | 10.8ABa | 11.3Ba | 9.72B | |||||||
0.4% FA | 3.69Bd | 6.74ABcd | 8.73ABcd | 11.3Abc | 15.7Aab | 17.1Aa | 10.5B | |||||||
0.6% FA | 2.81Bc | 3.23Bab | 3.34Bab | 4.20Bab | 5.47Bab | 6.76Ca | 4.30C | |||||||
NH3-N (% TN) | Control | 9.89Ac | 13.0Abc | 13.8Abc | 14.9Ab | 16.4Aab | 19.8Aa | 14.6A | 0.546 | <0.001 | <0.001 | <0.001 | 0.011 | <0.001 |
0.2% FA | 5.43Bb | 6.68Bb | 7.57Bab | 8.78ABa | 9.07Ca | 9.87Ba | 8.57B | |||||||
0.4% FA | 3.36Bd | 5.76Bcd | 7.44Bbc | 9.96ABb | 13.9Ba | 18.9Aa | 9.89B | |||||||
0.6% FA | 3.18Bc | 3.97Bc | 4.12Cbc | 5.12Babc | 6.39Dab | 7.03Ca | 4.97C | |||||||
Ethanol (% DM) | Control | 0.82Ad | 1.23Ac | 1.39Abc | 1.63Ab | 2.04Aa | 2.14Aa | 1.54A | 0.065 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
0.2% FA | 0.42Bb | 0.62Bab | 0.70BCab | 0.77Ca | 0.73Ca | 0.61Bab | 0.64C | |||||||
0.4% FA | 0.38BCd | 0.60Bcd | 0.82Bbc | 1.09Bb | 1.59Ba | 1.76Aa | 1.04B | |||||||
0.6% FA | 0.32Cd | 0.39Bcd | 0.44Cbc | 0.52Cab | 0.59Ca | 0.54Bab | 0.47D |
SEM, standard error of means; DM, dry matter; FW, fresh weight; NH3-N, ammonia nitrogen; TN, total nitrogen.
1) Control, no additive; 0.2% FA, 0.2% formic acid; 0.4% FA, 0.4% formic acid; 0.6% FA, 0.6% formic acid.
Items | Treatments1) | Ensiling days | Mean | SEM | p-value2) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|||||||||||||
3 | 6 | 9 | 15 | 30 | 60 | D | F | F-L | F-Q | D×F | ||||
NDF (% DM) | Control | 63.2Ad | 66.1Ac | 68.0Ab | 68.3Ab | 69.2Ab | 71.1Aa | 67.7A | 0.496 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
0.2% FA | 62.0ABb | 64.1Bab | 66.1ABa | 66.0ABa | 65.6Ba | 64.9Bab | 64.8B | |||||||
0.4% FA | 61.0Cd | 63.3Bcd | 64.9ABbcd | 66.5Aabc | 68.5Aab | 71.3Aa | 65.9AB | |||||||
0.6% FA | 59.8Ca | 58.6Cab | 58.4Cab | 58.7Bab | 58.4Cab | 57.9Cb | 58.6C | |||||||
ADF (% DM) | Control | 39.0c | 40.4bc | 41.5ab | 41.9ab | 42.0ab | 42.9a | 41.3 | 0.292 | 0.005 | 0.058 | 0.010 | 0.694 | 0.268 |
0.2% FA | 38.2 | 39.1 | 40.0 | 40.7 | 41.0 | 41.1 | 40.0 | |||||||
0.4% FA | 37.5d | 38.9cd | 39.3bc | 40.0bc | 42.0ab | 43.4a | 40.2 | |||||||
0.6% FA | 38.0 | 38.3 | 38.9 | 39.6 | 39.6 | 39.8 | 39.0 | |||||||
ADL (% DM) | Control | 5.33 | 5.47 | 5.42 | 5.55 | 5.58 | 5.87 | 5.54 | 0.061 | 0.544 | 0.423 | 0.250 | 0.649 | 0.916 |
0.2% FA | 5.22 | 5.35 | 5.23 | 5.31 | 5.29 | 5.35 | 5.29 | |||||||
0.4% FA | 5.15 | 5.27 | 5.29 | 5.39 | 5.57 | 5.97 | 5.44 | |||||||
0.6% FA | 5.11 | 5.18 | 5.22 | 5.33 | 5.29 | 5.36 | 5.25 | |||||||
Cellulose (% DM) | Control | 33.7b | 34.9ab | 36.1a | 36.3a | 36.5a | 37.0a | 35.8 | 0.265 | 0.007 | 0.072 | 0.013 | 0.727 | 0.131 |
0.2% FA | 33.0b | 33.7ab | 34.8ab | 35.4a | 35.7a | 35.7a | 34.7 | |||||||
0.4% FA | 32.4b | 33.6ab | 34.0ab | 34.7ab | 36.4a | 37.5a | 34.7 | |||||||
0.6% FA | 32.9 | 33.1 | 33.7 | 34.3 | 34.3 | 34.4 | 33.8 | |||||||
Hemicellulose (% DM) | Control | 24.2Ac | 25.7Abc | 26.5Aab | 26.4Aab | 27.2Aab | 28.2Aa | 26.4A | 0.399 | 0.730 | <0.001 | <0.001 | <0.001 | 0.139 |
0.2% FA | 23.8A | 25.1A | 26.1A | 25.3AB | 24.7B | 23.8B | 24.8B | |||||||
0.4% FA | 23.5Ac | 24.4Abc | 25.6Aab | 26.4Aab | 26.5Aab | 27.9Aa | 25.7A | |||||||
0.6% FA | 21.9Ba | 20.3Bab | 19.5Bab | 19.0Bab | 18.8Cab | 18.1Cb | 19.6C |
SEM, standard error of means; NDF, neutral detergent fibre; DM, dry matter; ADF, acid detergent fibre; ADL, acid detergent lignin.
1) Control, no additive; 0.2% FA, 0.2% formic acid; 0.4% FA, 0.4% formic acid; 0.6% FA, 0.6% formic acid.
Items | Treatments1) | SEM | p-value2) | |||||
---|---|---|---|---|---|---|---|---|
|
|
|||||||
Control | 0.2% FA | 0.4% FA | 0.6% FA | F | F-L | F-Q | ||
CP (% DM) | 5.89B | 6.27A | 6.05AB | 6.35A | 0.044 | 0.009 | 0.009 | 0.617 |
Ash (% DM) | 14.9A | 13.3B | 15.0A | 12.8B | 0.318 | 0.001 | 0.007 | 0.335 |
Potential gas production, b (mL) | 52.3C | 60.8B | 53.5C | 66.5A | 1.473 | <0.001 | <0.001 | 0.034 |
Gas production rate constant, c (mL/h) | 0.15 | 0.18 | 0.17 | 0.18 | 0.012 | 0.052 | 0.035 | 0.195 |
In vitro dry matter degradability (%) | 53.5 | 52.5 | 51.0 | 53.6 | 1.221 | 0.279 | 0.801 | 0.106 |
In vitro neutral detergent fibre degradability (%) | 51.4 | 49.5 | 50.7 | 49.0 | 2.430 | 0.852 | 0.755 | 0.787 |
In vitro acid detergent fibre degradability (%) | 44.5 | 45.3 | 44.8 | 45.6 | 2.838 | 0.725 | 0.418 | 0.701 |