Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs

Zhi, Ai-min; Feng, Ding-yuan; Zhou, Xiang-yan; Zou, Shi-geng; Huang, Zhi-yi; Zuo, Jian-jun; Ye, Hui; Zhang, Chang-ming; Dong, Ze-min; Liu, Zhun

doi:2008.21.8.1134

Article

Animal Reproduction and Physiology

Asian-Australasian Journal of Animal Sciences 2008;21(8): 1134-1142.
https://doi.org/10.5713/ajas.2008.80044 Published online July 1, 2008.

Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs

Ai-min Zhi, Ding-yuan Feng^*, Xiang-yan Zhou, Shi-geng Zou, Zhi-yi Huang, Jian-jun Zuo, Hui Ye, Chang-ming Zhang, Ze-min Dong, Zhun Liu

Correspondence: Ding-yuan Feng,

Abstract

Cationic amino acid transporter b0,+AT (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine b0,+AT. Based on the sequence of porcine b0,+AT deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine b0,+AT was isolated. The porcine b0,+AT cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse b0,+AT, respectively. Real-time RT-PCR indicated porcine b0,+AT transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest b0,+AT mRNA abundance while the muscle had the lowest (p<0.05). Along the longitudinal axis, the ileum had the highest b0,+ AT mRNA abundance while the colon had the lowest (p<0.05). The b0,+AT mRNA level was highest on day 7 and 90 in the duodenum (p<0.05). It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05). There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05). The strongest b0,+AT expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).

Keywords: Cationic Amino Acid Transporter; b0,+AT; SLC7A9; Ontogenetic Regulation