Effects of Testosterone, 17β-estradiol, and Progesterone on the Differentiation of Bovine Intramuscular Adipocytes

Oh, Young Sook; Cho, Sang Bum; Baek, Kyung Hoon; Choi, Chang Bon

doi:2005.18.11.1589

Article

Ruminant Nutrition and Forage Utilization

Asian-Australasian Journal of Animal Sciences 2005;18(11): 1589-1593.
https://doi.org/10.5713/ajas.2005.1589 Published online December 2, 2005.

Effects of Testosterone, 17β-estradiol, and Progesterone on the Differentiation of Bovine Intramuscular Adipocytes

Young Sook Oh, Sang Bum Cho, Kyung Hoon Baek, Chang Bon Choi

Abstract

The aim of this study was to investigate the effects of testosterone, 17棺-estradiol, and progesterone on the differentiation of bovine intramuscular adipocytes (BIA). Stromal-vascular (SV) cells were obtained from M. longissimus dorsi of 20 months old Korean (Hanwoo) steers, and were cultured in DMEM containing 5% FBS. The proliferated BIA were induced to differentiate with 0.25 M dexamethasone, 0.5 mM 1-methyl-3-isobutyl-xanthine and 10 g/ml insulin. During differentiation, the cells were treated with testosterone, 17棺-estradiol, and progesterone at concentrations of 10-10, 10-9, and 10-8 M, respectively, for 12 days. Regardless of its concentration, testosterone remarkably reduced lipid droplets in the cytosol of BIA. On the other hand, 17棺-estradiol and progesterone increased the accumulation of lipid droplets in BIA. Testosterone significantly (p<0.05) decreased GPDH activities with a dose-dependent pattern. 17棺-Estradiol treatment onto BIA during differentiation, however, increased GPDH activity showing the highest activity (11.3 nmol/mg protein/min) at 10-10 M. Treatment of BIA with progesterone also increased (p<0.05) GPDH activity with the highest activity (13.8 nmol/mg protein/min) at 10-9 M. In conclusion, the results in the current study suggest that testosterone inhibits differentiation of BIA by suppressing GPDH activity while 17棺-estradiol and progesterone have adverse effects.

Keywords: Testosterone; 17棺-estradiol; Progesterone; Bovine Adipocyte; GPDH