Characterization of Korean Cattle Keratin IV Gene

Yu, D. Y.; Yu, S. L.; Sang, B. C.; Kim, D. Y.

doi:2003.16.7.1055

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2003;16(7): 1055-1059.
https://doi.org/10.5713/ajas.2003.1055 Published online January 1, 2003.

Characterization of Korean Cattle Keratin IV Gene

D. Y. Yu, S. L. Yu, B. C. Sang, D. Y. Kim

Abstract

Keratins, the constituents of epithelial intermediate filaments, are precisely regulated in a tissue and development specific manner. There are two types of keratin in bovine. The type I is acidic keratin and the type II is neutral/basic keratin. 1.5 kb of 5'''' flanking sequence of Korean cattle Keratin IV gene, type II keratin (59 kDa), was cloned and sequenced. A symmetrical motif AApuCCAAA are located in a defined region upstream of the TATA box. Proximal SP1, AP1, E-box and CACC elements as the major determinants of transcription are identified. When it was compared to the bovine sequence from -600 bp to ATG upstream, the homology was 97% in nucleotide sequence. Several A and T sequences, located in the promoter region, are deleted in the Korean cattle. An expression vector consisted of Korean cattle Keratin IV gene promoter/SV40 large T antigen was transfected to HaCaT cell (Epithelial keratinocyte). The transformed HaCaT cells showed active proliferation when treated with PDGF (Platelet-derived growth factor) in 0.3% soft agar compared to control cells. These results indicate that Korean cattle Keratin IVgene promoter can be used as a promoter for transfection into epithelial cell.

Keywords: Keratinocyte; PDGF; Keratin IV Gene; Korean Cattle