Detection and Identification of β-lactamase, Enterotoxin and Other Exotoxins Genes of Staphylococcus aureus by PCR

Yoon, Y. H.; Kim, K. I.

doi:2003.16.3.425

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2003;16(3): 425-429.
https://doi.org/10.5713/ajas.2003.425 Published online January 1, 2003.

Detection and Identification of β-lactamase, Enterotoxin and Other Exotoxins Genes of Staphylococcus aureus by PCR

Y. H. Yoon, K. I. Kim

Abstract

Staphylococcus aureus is a major pathogen for cattle, causing various forms of subclinical and clinical mastitis and could be a causative agent of food poisoning, it produces various superantigenic exotoxins which have a great public health significance. A total of 72 S. aureus clinical isolates from dairy farms located in Kyunggi Province Korea were examined for the species identification by biochemical method, and for the detection of b-lactamase, enterotoxin and other exotoxins genes by PCR. The results of species identification by biochemical method agreed with those of PCR done with species specific primer STA-AU. b-lactamase is an enzyme closely associated with the resistance to antibiotic penicillin, which is an important means of treatment of mastitis, all the isolates were positive for the presence of genes encoding b-lactamase, which were reproduced in penicillin susceptibility disc assay. Six types of toxin genes, Staphylococcal enterotoxin (SE)A, SEB, SEC, SEE, toxic shock syndrome toxin (TSST-1) and exfoliative toxin A (ET A) were detected in 72 isolates by PCR associated genotypic method in this study, none of the isolates carried the genes for enterotoxin D (SED) and exfoliative toxin B (ETB). The occurrence rate of exotoxin genes rated as 12.5%, and the precision of the PCR identification results has been confirmed using the reference strains.

Keywords: Staphylococcus aureus; Enterotoxin; PCR; 棺-lactamase